Correction to: RNAi,what is its position in crop protection?     

Mezzetti  Bruno  Smagghe  Guy  Arpaia  Salvatore  Christiaens  Olivier  Dietz-Pfeilstetter  Antje  Jones  Huw  Kostov  Kaloyan  Sabbadini  Silvia  Opsahl-Sorteberg  Hilde-Gunn  Ventura  Vera  Taning  Clauvis Nji Tizi  Sweet  Jeremy 《Journal of pest science》2021,94(4):1555-1555

Journal of Pest Science - A correction to this paper has been published: https://doi.org/10.1007/s10340-021-01393-0  相似文献   

Targeting a coatomer protein complex-I gene via RNA interference results in effective lethality in the pollen beetle Brassicogethes aeneus     

Willow  Jonathan  Sulg  Silva  Taning  Clauvis Nji Tizi  Silva  Ana Isabel  Christiaens  Olivier  Kaasik  Riina  Prentice  Katterinne  Lövei  Gabor L.  Smagghe  Guy  Veromann  Eve 《Journal of pest science》2021,94(3):703-712

The pollen beetle Brassicogethes aeneus is a serious pest of oilseed rape (Brassica napus) in Europe. Management of this pest has grown difficult due to B. aeneus’s development of resistance to pyrethroid insecticides, as well as the pressure to establish control strategies that minimise the impact on nontarget organisms. RNA interference represents a nucleotide sequence-based, and thus potentially species-specific, approach to agricultural pest control. The present study examined the efficacy of targeting the coatomer gene coatomer subunit alpha (αCOP), via both microinjection and dietary exposure to exogenous complementary dsRNA, on αCOP-silencing and subsequent mortality in B. aeneus. Beetles injected with dsRNA targeting αCOP (at 0.14 µg/mg) showed 88% and 100% mortality at 6 and 10 days post-injection, respectively; where by the same time after dietary exposure, 43%–89% mortality was observed in the 3 µg dsRNA/µL treatment, though the effect was concentration-dependent. Thus, the effect was significant for both delivery routes. In working towards RNA-based management of B. aeneus, future studies should include αCOP as a target of interest.

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